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Scale‐down of continuous protein producing Saccharomyces cerevisiae cultivations using a two‐compartment system
Author(s) -
Risager Wright Naia,
Rønnest Nanna Petersen,
Thykaer Jette
Publication year - 2015
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.2184
Subject(s) - heterologous , saccharomyces cerevisiae , substrate (aquarium) , bioreactor , biology , biochemistry , scale (ratio) , industrial biotechnology , fermentation , continuous production , biochemical engineering , microbiology and biotechnology , chemistry , yeast , environmental science , ecology , botany , environmental engineering , gene , physics , quantum mechanics , engineering
In the biotechnological industry, economic decisions in investment are typically based on laboratory‐scale experiments. Scale‐down as a tool is therefore of high industrial importance to transfer the processes into larger production scale without loss in performance. In this study, large‐scale prolonged continuous cultivations with a heterologous protein producing Saccharomyces cerevisiae strain have been scaled‐down to a two‐compartment scale‐down reactor system. The effects of glucose, pH, and oxygen concentration gradients have been investigated by comparison with corresponding 300 mL standard continuous cultivations. It was found that substrate gradients within a limited range result in increased productivity of the heterologous protein under regulation of glycolytic TPI promoter and delay the decrease of protein and trehalose production during continuous cultivation. Based on these results, it is argued that introduction of variations in substrate concentration can be beneficial for industrial continuous cultivations. © 2015 American Institute of Chemical Engineers Biotechnol. Prog. , 32:152–159, 2016