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High‐titer production and strong antimicrobial activity of sophorolipids from Rhodotorula bogoriensis
Author(s) -
Solaiman Daniel K.Y.,
Ashby Richard D.,
Crocker Nicole V.
Publication year - 2015
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.2101
Subject(s) - fermentation , chemistry , bioreactor , antimicrobial , high performance liquid chromatography , food science , biochemistry , chromatography , organic chemistry
Rhodotorula bogoriensis produces sophorolipids (SLs) that contain 13‐hydroxydocosanoic acid (OH‐C 22 ) as the lipid moiety. A systematic study was conducted to further understand the fermentative production of SLs containing OH‐C 22 (C 22 ‐SL) by R. bogoriensis . Shake‐flask studies showed that R. bogoriensis consumed glucose at a slow pace. HPLC analysis of the C 22 ‐SL products from shake‐flask fermentations at different glucose concentrations showed a correlation between glucose depletion and the extent of C 22 ‐SL deacetylation. A large‐scale bioreactor fermentation resulted in the isolation of C 22 ‐SL at a volumetric product yield of 51 g/L. HPLC analysis of C 22 ‐SL product from the bioreactor fermentation corroborated the finding that glucose depletion correlated with extensive deacetylation of C 22 ‐SL. The antimicrobial activity of C 22 ‐SL was established for the first time to be stronger than the C 18 ‐SL from Candida bombicola against Propionibacterium acnes in a plate assay. © 2015 American Institute of Chemical Engineers Biotechnol. Prog ., 31:867–874, 2015