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Expression of a collagen‐binding domain fusion protein: Effect of amino acid supplementation, inducer type, and culture conditions
Author(s) -
Fruchtl McKinzie,
Sakon Joshua,
Beitle Robert
Publication year - 2015
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.2048
Subject(s) - inducer , lactose , lac operon , fusion protein , amino acid , chemistry , escherichia coli , fermentation , biochemistry , isopropyl , recombinant dna , organic chemistry , gene
Collagen binding domain fusion proteins are of significant importance because of their potential as therapeutic biomaterials. In this paper, we investigate the production of such therapeutic proteins via fermentation of Escherichia coli on both an undefined medium and a defined medium. Defined media with amino acid supplementation provided higher amounts of therapeutic protein than undefined media with no supplementation. Additionally, utilizing lactose instead of isopropyl‐β‐ d ‐thio‐galactoside (IPTG) for induction and extending batch time yielded higher amounts of the model therapeutic. © 2014 American Institute of Chemical Engineers Biotechnol. Prog. , 31:503–509, 2015