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Biosynthesis of copper nanoparticles and its effect on actively dividing cells of mitosis in Allium cepa
Author(s) -
Nagaonkar Dipali,
Shende Sudhir,
Rai Mahendra
Publication year - 2015
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.2040
Subject(s) - anaphase , allium , mitotic index , copper , mitosis , metaphase , nanoparticle , context (archaeology) , telophase , chemistry , botany , biology , microbiology and biotechnology , biochemistry , nanotechnology , cell cycle , materials science , chromosome , organic chemistry , cell , gene , paleontology
Nanobiotechnological application of copper nanoparticles has paved the way for advancement in agriculture owing to its bactericidal and fungicidal activities. Recently, researchers have focussed on bioinspired synthesis of copper nanoparticles as a viable alternative to existing physicochemical techniques. For the commercialization of nanocopper, the toxicity evaluation is a major issue. In this context, Citrus medica (L.) fruit extract‐mediated copper nanoparticles were synthesized and its different concentrations (10, 20, 40, 60, 80, and 100 µg mL −1 ) were evaluated for its effect on actively dividing cells of Allium cepa. The study clearly revealed that copper nanoparticles increased mitotic index up to the concentration of 20 µg mL −1 . In addition, a gradual decline in mitotic index and increase in abnormality index was observed as the concentration of copper nanoparticles and treatment duration were increased. Aberrations in chromosomal behavior such as sticky and disturbed chromosomes in metaphase and anaphase, c‐metaphase, bridges, laggard, disturbed telophase, and vacuolated nucleus were also observed. © 2015 American Institute of Chemical Engineers Biotechnol. Prog. , 31:557–565, 2015