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HS‐SPME‐GC‐FID method for detection and quantification of B acillus cereus ATCC 10702 mediated 2‐acetyl‐1‐pyrroline
Author(s) -
Deshmukh Yogita,
Khare Puja,
Patra D. D.,
Nadaf Altafhusain B.
Publication year - 2014
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.1989
Subject(s) - cereus , bacillus cereus , chromatography , chemistry , octanal , hexanal , aroma compound , aroma , detection limit , extraction (chemistry) , incubation , gram , food science , bacteria , biochemistry , biology , genetics
A rapid micro‐scale solid‐phase micro‐extraction (SPME) procedure coupled with gas‐chromatography with flame ionized detector (GC‐FID) was used to extract parts per billion levels of a principle basmati aroma compound “2‐acetyl‐1‐pyrroline” (2‐AP) from bacterial samples. In present investigation, optimization parameters of bacterial incubation period, sample weight, pre‐incubation time, adsorption time, and temperature, precursors and their concentrations has been studied. In the optimized conditions, detection of 2‐AP produced by Bacillus cereus ATCC10702 using only 0.5 g of sample volume was 85 μg/kg. Along with 2‐AP, 15 other compounds produced by B. cereus were also reported out of which 14 were reported for the first time consisting mainly of (E)−2‐hexenal, pentadecanal, 4‐hydroxy‐2‐butanone, n ‐hexanal, 2–6‐nonadienal, 3‐methoxy‐2(5 H ) furanone and 2‐acetyl‐1‐pyridine and octanal. High recovery of 2‐AP (87 %) from very less amount of B. cereus samples was observed. The method is reproducible fast and can be used for detection of 2‐AP production by B. cereus . © 2014 American Institute of Chemical Engineers Biotechnol. Prog ., 30:1356–1363, 2014