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Assessment of mass transfer and mixing in rigid lab‐scale disposable bioreactors at low power input levels
Author(s) -
van Eikenhorst Gerco,
Thomassen Yvonne E.,
van der Pol Leo A.,
Bakker Wilfried A. M.
Publication year - 2014
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.1981
Subject(s) - bioreactor , aeration , mixing (physics) , mass transfer , mass transfer coefficient , microcarrier , power consumption , materials science , scale up , suspension (topology) , chromatography , power (physics) , suspension culture , pulp and paper industry , process engineering , chemistry , environmental science , mathematics , thermodynamics , physics , engineering , cell culture , biology , cell , homotopy , pure mathematics , biochemistry , organic chemistry , quantum mechanics , classical mechanics , genetics
Mass transfer, mixing times and power consumption were measured in rigid disposable stirred tank bioreactors and compared to those of a traditional glass bioreactor. The volumetric mass transfer coefficient and mixing times are usually determined at high agitation speeds in combination with sparged aeration as used for single cell suspension and most bacterial cultures. In contrast, here low agitation speeds combined with headspace aeration were applied. These settings are generally used for cultivation of mammalian cells growing adherent to microcarriers. The rigid disposable vessels showed similar engineering characteristics compared to a traditional glass bioreactor. On the basis of the presented results appropriate settings for adherent cell culture, normally operated at a maximum power input level of 5 W m −3 , can be selected. Depending on the disposable bioreactor used, a stirrer speed ranging from 38 to 147 rpm will result in such a power input of 5 W m −3 . This power input will mix the fluid to a degree of 95% in 22 ± 1 s and produce a volumetric mass transfer coefficient of 0.46 ± 0.07 h −1 . © 2014 American Institute of Chemical Engineers Biotechnol. Prog ., 30:1269–1276, 2014