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Optimization and scale‐up of oligonucleotide synthesis in packed bed reactors using computational fluid dynamics modeling
Author(s) -
Wolfrum Christian,
Josten Andre,
Götz Peter
Publication year - 2014
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.1966
Subject(s) - computational fluid dynamics , packed bed , oligonucleotide , scale (ratio) , kinetics , scale up , scale model , chemistry , thermodynamics , biological system , mechanics , chromatography , engineering , physics , biology , dna , biochemistry , quantum mechanics , classical mechanics , aerospace engineering
A computational fluid dynamics (CFD) model for the analysis of oligonucleotide synthesis in packed bed reactors was developed and used to optimize the scale up of the process. The model includes reaction kinetics data obtained under well defined conditions comparable to the situation in the packed bed. The model was validated in terms of flow conditions and reaction kinetics by comparison with experimental data. Experimental validation and the following model parameter studies by simulation were performed on the basis of a column with 0.3 g oligonucleotide capacity. The scale‐up studies based on CFD modelling were calculated on a 440 g scale (oligonucleotide capacity). © 2014 American Institute of Chemical Engineers Biotechnol. Prog ., 30:1048–1056, 2014