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Gel filtration chromatography as a method for removing bacterial endotoxin from antibody preparations
Author(s) -
London Anne Serdakowski,
Mackay Kasey,
Lihon Michael,
He Yaqin,
Alabi Busola Ruth
Publication year - 2014
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.1961
Subject(s) - chromatography , size exclusion chromatography , chemistry , filtration (mathematics) , antibody , microbiology and biotechnology , biology , biochemistry , enzyme , immunology , statistics , mathematics
The removal of bacterial endotoxins from biological samples is critical to avoid the potentially fatal pyrogenic response possible when introduced to mammalian systems. Endotoxins have a variety of specific characteristics that can be exploited to target their isolation and subsequent removal, but one that has not been extensively characterized is their difference in size from that of monoclonal antibodies. Here, we present a study which utilizes gel filtration chromatography as a method for endotoxin removal from both aggregated and nonaggregated antibody preparations, outlining a mechanistically simple method for removal of this impurity. © 2014 American Institute of Chemical Engineers Biotechnol. Prog ., 30:1497–1501, 2014