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A novel DAD type and folic acid conjugated fluorescent monomer as a targeting probe for imaging of folate receptor overexpressed cells
Author(s) -
Ekiz Kanik Fulya,
Ag Didem,
Seleci Muharrem,
Barlas Firat Baris,
Kesik Melis,
Hizalan Gonul,
Akpinar Hava,
Timur Suna,
Toppare Levent
Publication year - 2014
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.1928
Subject(s) - folate receptor , monomer , fluorescence , chemistry , conjugated system , amine gas treating , cytotoxicity , folic acid , acceptor , fluorescence microscope , surface modification , fourier transform infrared spectroscopy , biochemistry , biophysics , in vitro , cancer cell , organic chemistry , polymer , biology , cancer , medicine , genetics , physics , quantum mechanics , condensed matter physics
We describe a modification and post‐functionalization technique for a donor–acceptor–donor type monomer; 6‐(4,7‐bis(2,3‐dihydrothieno[3,4‐ b ][1,4]dioxin‐5‐yl)‐2 H ‐benzo[ d ][1,2, 3]triazol‐2‐yl)hexan‐1‐amine. Folic acid was attached to the fluorescent structure. The conjugation was confirmed via NMR and Fourier transform infrared analyses. Cytotoxicity was investigated and the comparison of association of targeted monomeric structures in tumor cells was monitored via fluorescence microscopy. © 2014 American Institute of Chemical Engineers Biotechnol. Prog. , 30:952–959, 2014