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Production of recombinant protein in Escherichia coli cultured in extract from waste product alga, Ulva lactuca
Author(s) -
Rechtin Tammy M.,
Hurst Matthew,
Potts Tom,
Hestekin Jamie,
Beitle Robert,
McLaughlin John,
May Peter
Publication year - 2014
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.1924
Subject(s) - ulva lactuca , escherichia coli , recombinant dna , lactuca , food science , green fluorescent protein , biology , botany , algae , biochemistry , chemistry , microbiology and biotechnology , gene
This study examined the potential for waste product alga, Ulva lactuca , to serve as a media component for recombinant protein production in Escherichia coli . To facilitate this investigation, U. lactuca harvested from Jamaica Bay was dried, and nutrients acid extracted for use as a growth media. The E. coli cell line BL21(DE3) was used to assess the effects on growth and production of recombinant green fluorescent protein (GFP). This study showed that media composed of acid extracts without further nutrient addition maintained E. coli growth and recombinant protein production. Extracts made from dried algae lots less than six‐months‐old were able to produce two‐fold more GFP protein than traditional Lysogeny Broth media. © 2014 American Institute of Chemical Engineers Biotechnol. Prog ., 30:784–789, 2014