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Different types of aqueous two‐phase systems for biomolecule and bioparticle extraction and purification
Author(s) -
Molino João Vitor Dutra,
Viana Marques Daniela de Araújo,
Júnior Adalberto Pessoa,
Mazzola Priscila Gava,
Gatti Maria Silvia Viccari
Publication year - 2013
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.1792
Subject(s) - biomolecule , downstream processing , bottleneck , extraction (chemistry) , biochemical engineering , aqueous solution , chemistry , chromatography , microbiology and biotechnology , nanotechnology , process engineering , computer science , materials science , biology , engineering , organic chemistry , embedded system
Upstream improvements have led to significant advances in the productivity of biomolecules and bioparticles. Today, downstream processes are the bottleneck in the production of some biopharmaceuticals, a change from previous years. Current purification platforms will reach their physical limits at some point, indicating the need for new approaches. This article reviews an alternative method to extract and purify biomolecules/bioparticles named aqueous two‐phase system (ATPS). Biocompatibility and readiness to scale up are some of the ATPS characteristics. We also discuss some of ATPS applications in the biotechnology field. © 2013 American Institute of Chemical Engineers Biotechnol. Prog ., 29:1343–1353, 2013