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Properties of Geobacillus stearothermophilus levansucrase as potential biocatalyst for the synthesis of levan and fructooligosaccharides
Author(s) -
Inthanavong Lotthida,
Tian Feng,
Khodadadi Maryam,
Karboune Salwa
Publication year - 2013
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.1788
Subject(s) - levansucrase , geobacillus stearothermophilus , chemistry , maltose , thermophile , fructose , raffinose , sucrose , glycerol , hydrolysis , substrate (aquarium) , catalysis , biochemistry , chromatography , enzyme , bacteria , bacillus subtilis , biology , genetics , ecology
The production of levansucrase (LS) by thermophilic Geobacillus stearothermophilus was investigated. LS production was more effective in the presence of sucrose (1%, w/v) than fructose, glucose, glycerol or raffinose. The results ( T op 57°C; stable for 6 h at 47°C) indicate the high stability of the transfructosylation activity of G. stearothermophilus LS as compared with LSs from other microbial sources. Contrary to temperature, the pH had a significant effect on the selectivity of G. stearothermophilus LS‐catalyzed reaction, favoring the transfructosylation reaction in the pH range of 6.0–6.5. The kinetic parameter study revealed that the catalytic efficiency of transfructosylation activity was higher as compared with the hydrolytic one. In addition to levan, G . stearothermophilus LS synthesized fructooligosaccharides in the presence of sucrose as the sole substrate. The results also demonstrated the wide acceptor specificity of G . stearothermophilus LS with maltose being the best fructosyl acceptor. This study is the first on the catalytic properties and the acceptor specificity of LS from G. stearothermophilus . © 2013 American Institute of Chemical Engineers Biotechnol. Prog ., 29:1405–1415, 2013