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Synergistic coordination of polyethylene glycol with ClpB/DnaKJE bichaperone for refolding of heat‐denatured malate dehydrogenase
Author(s) -
Nian Rui,
Kim Duck Sang,
Tan Lihan,
Kim ChanWha,
Choe WooSeok
Publication year - 2009
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.175
Subject(s) - clpb , polyethylene glycol , chemistry , peg ratio , malate dehydrogenase , chaperone (clinical) , ethylene glycol , biochemistry , biophysics , enzyme , organic chemistry , heat shock protein , biology , medicine , finance , pathology , economics , gene
The use of polyethylene glycol (PEG) as a refolding additive to a refolding cocktail comprising the molecular bichaperone ClpB and DnaKJE significantly enhances chaperone‐mediated refolding of heat‐denatured malate dehydrogenase (MDH). The critical factor to affect the refolding yield is the time point of introducing PEG to the refolding cocktail. The refolding efficiency reached approximately 90% only when PEG was added at the beginning of refolding reaction. The synergistic coordination of an inexpensive refolding additive PEG with the ClpB/DnaKJE bichaperone system may provide an economical route to further enhance the efficacy of ClpB/DnaKJE refolding cocktail approach, facilitating its implementation in large‐scale refolding processes. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009