Premium
Development of large engineered cartilage constructs from a small population of cells
Author(s) -
Brenner Jillian M.,
Kunz Manuela,
Tse Man Yat,
Winterborn Andrew,
Bardana Davide D.,
Pang Stephen C.,
Waldman Stephen D.
Publication year - 2013
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.1670
Subject(s) - cartilage , hyaline cartilage , chondrogenesis , tissue engineering , chondrocyte , regeneration (biology) , biomedical engineering , hyaline , matrix (chemical analysis) , population , articular cartilage , anatomy , biology , chemistry , materials science , microbiology and biotechnology , pathology , medicine , osteoarthritis , composite material , alternative medicine , environmental health
Confronted with articular cartilage's limited capacity for self‐repair, joint resurfacing techniques offer an attractive treatment for damaged or diseased tissue. Although tissue engineered cartilage constructs can be created, a substantial number of cells are required to generate sufficient quantities of tissue for the repair of large defects. As routine cell expansion methods tend to elicit negative effects on chondrocyte function, we have developed an approach to generate phenotypically stable, large‐sized engineered constructs (≥3 cm 2 ) directly from a small amount of donor tissue or cells (as little as 20,000 cells to generate a 3 cm 2 tissue construct). Using rabbit donor tissue, the bioreactor‐cultivated constructs were hyaline‐like in appearance and possessed a biochemical composition similar to native articular cartilage. Longer bioreactor cultivation times resulted in increased matrix deposition and improved mechanical properties determined over a 4 week period. Additionally, as the anatomy of the joint will need to be taken in account to effectively resurface large affected areas, we have also explored the possibility of generating constructs matched to the shape and surface geometry of a defect site through the use of rapid‐prototyped defect tissue culture molds. Similar hyaline‐like tissue constructs were developed that also possessed a high degree of shape correlation to the original defect mold. Future studies will be aimed at determining the effectiveness of this approach to the repair of cartilage defects in an animal model and the creation of large‐sized osteochondral constructs. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2013