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Application of aurintricarboxylic acid for the adherence of mouse P19 neurons and primary hippocampal neurons to noncoated surface in serum‐free culture
Author(s) -
Lee TsungYih,
Chen WenShin,
Huang YungAn,
Liu TingWei,
Hwang Eric,
Tseng ChingPing
Publication year - 2012
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.1638
Subject(s) - aurintricarboxylic acid , hippocampal formation , chinese hamster ovary cell , neurite , retinoic acid , cell culture , biology , neuron , embryonal carcinoma , microbiology and biotechnology , chemistry , biochemistry , neuroscience , in vitro , cellular differentiation , apoptosis , genetics , programmed cell death , gene
Dissociated primary neuron culture has been the most widely used model systems for neuroscience research. Most of these primary neurons are cultured on adhesion matrix‐coated surface to provide a proper environment for cell anchorage under serum‐free conditions. In this study, we provide an alternative technique to promote the adhesions of these neurons using aurintricarboxylic acid (ATA), a nonpeptide compound, without surface manipulations. We first demonstrated that ATA could promote Chinese hamster ovary cell attachment and proliferation in serum‐free medium in a dosage‐dependent manner. We later showed that ATA significantly enhanced the attachment of the retinoic acid differentiated P19 mouse embryonal carcinoma (P19) neurons, with an optimal concentration around 30 μg/mL. A similar result was seen in primary hippocampal neurons, with an optimal ATA concentration around 15 μg/mL. Further morphological assessments revealed that the average neurite length and neuronal polarization were almost identical to that obtained using a conventional method with poly‐ L ‐lysine surface. The advantages of using the ATA treatment technique for immunochemical analysis are discussed. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012