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Neural stimulation on human bone marrow‐derived mesenchymal stem cells by extremely low frequency electromagnetic fields
Author(s) -
Cho Hyunjin,
Seo YoungKwon,
Yoon HeeHoon,
Kim SooChan,
Kim SungMin,
Song KyeYong,
Park JungKeug
Publication year - 2012
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.1607
Subject(s) - mesenchymal stem cell , neural stem cell , stem cell , microbiology and biotechnology , nestin , induced pluripotent stem cell , biology , neurosphere , stimulation , neuroepithelial cell , adult stem cell , cellular differentiation , immunology , neuroscience , embryonic stem cell , biochemistry , gene
Adult stem cells are considered multipotent. Especially, human bone marrow‐derived mesenchymal stem cells (hBM‐MSCs) have the potential to differentiate into nerve type cells. Electromagnetic fields (EMFs) are widely distributed in the environment, and recently there have been many reports on the biological effects of EMFs. hBM‐MSCs are weak and sensitive pluripotent stem cells, therefore extremely low frequency‐electromagnetic fields (ELF‐EMFs) could be affect the changes of biological functions within the cells. In our experiments, ELF‐EMFs inhibited the growth of hBM‐MSCs in 12 days exposure. Their gene level was changed and expression of the neural stem cell marker like nestin was decreased but the neural cell markers like MAP2, NEUROD1, NF‐L, and Tau were induced. In immunofluorescence study, we confirmed the expression of each protein of neural cells. And also both oligodendrocyte and astrocyte related proteins like O4 and GFAP were expressed by ELF‐EMFs. We suggest that EMFs can induce neural differentiation in BM‐MSCs without any chemicals or differentiation factors. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012