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Biotransformation of 2,6‐diaminopurine nucleosides by immobilized Geobacillus stearothermophilus
Author(s) -
De Benedetti Eliana C.,
Rivero Cintia W.,
Britos Claudia N.,
Lozano Mario E.,
Trelles Jorge A.
Publication year - 2012
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.1602
Subject(s) - geobacillus stearothermophilus , biotransformation , thermophile , chemistry , bioprocess , hypoxanthine , nucleoside , prodrug , oligonucleotide , combinatorial chemistry , biochemistry , biology , enzyme , dna , paleontology
An efficient and green bioprocess to obtain 2,6‐diaminopurine nucleosides using thermophilic bacteria is herein reported. Geobacillus stearothermophilus CECT 43 showed a conversion rate of 90 and 83% at 2 h to obtain 2,6‐diaminopurine‐2′‐deoxyriboside and 2,6‐diaminopurine riboside, respectively. The selected biocatalyst was successfully stabilized in an agarose matrix and used to produce up to 23.4 g of 2,6‐diaminopurine‐2′‐deoxyriboside in 240 h of process. These nucleoside analogues can be used as prodrug precursors or in antisense oligonucleotide synthesis. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012
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