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In situ Raman spectroscopy for simultaneous monitoring of multiple process parameters in mammalian cell culture bioreactors
Author(s) -
Whelan Jessica,
Craven Stephen,
Glen Brian
Publication year - 2012
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.1590
Subject(s) - bioprocess , bioreactor , process analytical technology , partial least squares regression , calibration , analyte , in situ , raman spectroscopy , chemistry , chromatography , analytical chemistry (journal) , biological system , computer science , mathematics , biology , chemical engineering , engineering , statistics , physics , organic chemistry , optics , machine learning
In this study, the application of Raman spectroscopy to the simultaneous quantitative determination of glucose, glutamine, lactate, ammonia, glutamate, total cell density (TCD), and viable cell density (VCD) in a CHO fed‐batch process was demonstrated in situ in 3 L and 15 L bioreactors. Spectral preprocessing and partial least squares (PLS) regression were used to correlate spectral data with off‐line reference data. Separate PLS calibration models were developed for each analyte at the 3 L laboratory bioreactor scale before assessing its transferability to the same bioprocess conducted at the 15 L pilot scale. PLS calibration models were successfully developed for all analytes bar VCD and transferred to the 15 L scale. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012