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3,4‐dihydroxy‐ L ‐phenylalanine as a cell adhesion molecule in serum‐free cell culture
Author(s) -
La WanGeun,
Bhang Suk Ho,
Shin JungYoun,
Yoon Hee Hun,
Park Jooyeon,
Yang Hee Seok,
Yu SeungHo,
Sung YungEun,
Kim ByungSoo
Publication year - 2012
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.1560
Subject(s) - adhesion , fibronectin , cell adhesion , cell adhesion molecule , cell culture , phenylalanine , molecule , chemistry , viability assay , adhesive , cell , biochemistry , microbiology and biotechnology , biology , amino acid , organic chemistry , genetics , layer (electronics)
In this article, we examined the feasibility of using 3,4‐dihydroxy‐ L ‐phenylalanine (DOPA) as a cell adhesion molecule in serum‐free cultures of anchorage‐dependent mammalian cells. DOPA is a critical, functional element in mussel adhesive proteins and is known to bind strongly to various natural or synthetic materials. DOPA coating on culture plates was confirmed using X‐ray photoelectron spectroscopy and energy‐dispersive spectroscopy. Human dermal fibroblasts (HDFs) were cultured on DOPA‐coated, fibronectin‐coated, or no material‐coated culture plates in serum‐free medium. HDFs cultured on DOPA showed the highest cell adhesion ratio, spreading, and viability but the lowest apoptotic activity. Therefore, DOPA may be a useful cell‐adhesion molecule for serum‐free culture. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 28: 1055–1060, 2012