Premium
Automated methods for accurate determination of the critical velocity of packed bed chromatography
Author(s) -
Chang YuChih,
Gerontas Spyridon,
TitchenerHooker Nigel J.
Publication year - 2012
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.1544
Subject(s) - packed bed , pressure drop , chromatography , column (typography) , flow (mathematics) , process (computing) , drop (telecommunication) , flow velocity , automated method , critical ionization velocity , accuracy and precision , chemistry , computer science , analytical chemistry (journal) , mechanics , mathematics , artificial intelligence , statistics , physics , telecommunications , frame (networking) , operating system
Knowing the critical velocity ( u crit ) of a chromatography column is an important part of process development as it allows the optimization of chromatographic flow conditions. The conventional flow step method for determining u crit is prone to error as it depends heavily on human judgment. In this study, two automated methods for determining u crit have been developed: the automatic flow step (AFS) method and the automatic pressure step (APS) method. In the AFS method, the column pressure drop is monitored upon application of automated incremental increases in flow velocity, whereas in the APS method the flow velocity is monitored upon application of automated incremental increases in pressure drop. The APS method emerged as the one with the higher levels of accuracy, efficiency and ease of application having the greater potential to assist defining the best operational parameters of a chromatography column. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012