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Plant‐produced human growth hormone shows biological activity in a rat model
Author(s) -
Rabindran Shailaja,
Stevenson Natalie,
Roy Gourgopal,
Fedorkin Oleg,
Skarjinskaia Marina,
Ensley Burt,
Yusibov Vidadi
Publication year - 2009
Publication title -
biotechnology progress
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.572
H-Index - 129
eISSN - 1520-6033
pISSN - 8756-7938
DOI - 10.1002/btpr.130
Subject(s) - nicotiana benthamiana , recombinant dna , human growth hormone , plant growth , plant hormone , biology , population , growth hormone , hormone , biological activity , microbiology and biotechnology , in vitro , virus , biochemistry , botany , virology , medicine , environmental health , gene
Plants have been shown to be efficient systems for expressing a wide range of recombinant proteins from various origins. Here, using a plant virus‐based expression vector to produce human growth hormone (hGH) in Nicotiana benthamiana plants, we demonstrate, for the first time, that the plant‐produced hGH (pphGH) is biologically active in a hypophysectomized rat model. We observed an average weight gain of ∼17 g per animal in a group of 10 animals that were injected subcutaneously with pphGH with 60 μg/dose for 10 days. With the increasing demand for hGH, accompanied with the need to make this recombinant protein available to a wider population at a more reasonable cost, plants provide a feasible alternative to current production platforms. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009