Open AccessFluidic integrated 3D bioprinting system to sustain cell viability towards larynx fabrication
Author(s) -
Park Hae Sang,
Lee Ji Seung,
Kim ChangBeom,
Lee KwangHo,
Hong InSun,
Jung Harry,
Lee Hanna,
Lee Young Jin,
Ajiteru Olatunji,
Sultan Md Tipu,
Lee Ok Joo,
Kim Soon Hee,
Park Chan Hum
Publication year - 2023
Publication title -
bioengineering and translational medicine
Language(s) - English
Resource type - Journals
ISSN - 2380-6761
DOI - 10.1002/btm2.10423
Subject(s) - fluidics , 3d bioprinting , fabrication , microfluidics , larynx , nanotechnology , materials science , engineering , biomedical engineering , tissue engineering , medicine , aerospace engineering , anatomy , pathology , alternative medicine
Herein, we report the first study to create a three‐dimensional (3D) bioprinted artificial larynx for whole‐laryngeal replacement. Our 3D bio‐printed larynx was generated using extrusion‐based 3D bioprinter with rabbit's chondrocyte‐laden gelatin methacryloyl (GelMA)/glycidyl‐methacrylated hyaluronic acid (GMHA) hybrid bioink. We used a polycaprolactone (PCL) outer framework incorporated with pores to achieve the structural strength of printed constructs, as well as to provide a suitable microenvironment to support printed cells. Notably, we established a novel fluidics supply (FS) system that simultaneously supplies basal medium together with a 3D bioprinting process, thereby improving cell survival during the printing process. Our results showed that the FS system enhanced post‐printing cell viability, which enabled the generation of a large‐scale cell‐laden artificial laryngeal framework. Additionally, the incorporation of the PCL outer framework with pores and inner hydrogel provides structural stability and sufficient nutrient/oxygen transport. An animal study confirmed that the transplanted 3D bio‐larynx successfully maintained the airway. With further development, our new strategy holds great potential for fabricating human‐scale larynxes with in vivo‐like biological functions for laryngectomy patients.