Open AccessFinger stick blood test to assess postvaccination SARS‐CoV ‐2 neutralizing antibody response against variants
Author(s) -
Lim Sing Mei,
Cheng Hoi Lok,
Jia Huan,
Kongsuphol Patthara,
DO Shunmuganathan Bhuvaneshwari,
Chen Ming Wei,
Ng Say Yong,
Gao Xiaohong,
Turaga Shuvan Prashant,
Heussler Sascha P.,
Somani Jyoti,
Sengupta Sharmila,
Tay Dousabel M. Y.,
McBee Megan E.,
Young Barnaby E.,
MacAry Paul A.,
Sikes Hadley D.,
Preiser Peter R.
Publication year - 2022
Publication title -
bioengineering and translational medicine
Language(s) - English
Resource type - Journals
ISSN - 2380-6761
DOI - 10.1002/btm2.10293
Subject(s) - vaccination , medicine , covid-19 , neutralizing antibody , serology , population , antibody , virology , pandemic , immunology , antibody response , test (biology) , biology , paleontology , disease , environmental health , outbreak , infectious disease (medical specialty)
There is clinical need for a quantifiable point‐of‐care (PoC) SARS‐CoV‐2 neutralizing antibody (nAb) test that is adaptable with the pandemic's changing landscape. Here, we present a rapid and semi‐quantitative nAb test that uses finger stick or venous blood to assess the nAb response of vaccinated population against wild‐type (WT), alpha, beta, gamma, and delta variant RBDs. It captures a clinically relevant range of nAb levels, and effectively differentiates prevaccination, post first dose, and post second dose vaccination samples within 10 min. The data observed against alpha, beta, gamma, and delta variants agrees with published results evaluated in established serology tests. Finally, our test revealed a substantial reduction in nAb level for beta, gamma, and delta variants between early BNT162b2 vaccination group (within 3 months) and later vaccination group (post 3 months). This test is highly suited for PoC settings and provides an insightful nAb response in a postvaccinated population.