Open AccessChitosan nanofiber biocomposites for potential wound healing applications: Antioxidant activity with synergic antibacterial effect
Author(s) -
Bagheri Mitra,
Validi Majid,
Gholipour Abolfazl,
Makvandi Pooyan,
Sharifi Esmaeel
Publication year - 2022
Publication title -
bioengineering and translational medicine
Language(s) - English
Resource type - Journals
ISSN - 2380-6761
DOI - 10.1002/btm2.10254
Subject(s) - chitosan , wound healing , antibacterial activity , nanofiber , staphylococcus aureus , fibroblast , chemistry , pseudomonas aeruginosa , microbiology and biotechnology , viability assay , bacteria , materials science , medicine , nanotechnology , cell , biology , immunology , biochemistry , in vitro , genetics
Bacterial wound infection is one of the most common nosocomial infections. The unnecessary employment of antibiotics led to raising the growth of antibiotic‐resistant bacteria. Accordingly, alternative armaments capable of accelerating wound healing along with bactericidal effects are urgently needed. Considering this, we fabricated chitosan (CS)/polyethylene oxide (PEO) nanofibers armed with antibacterial silver and zinc oxide nanoparticles. The nanocomposites exhibited a high antioxidant effect and antibacterial activity against Staphylococcus aureus , Escherichia coli , and Pseudomonas aeruginosa . Besides, based on the results of the cell viability assays, the optimum concentration of ZnONPs and AgNPs in the nanofibrous mats is 0.2% w/v and 0.08% w/v respectively and had no cytotoxicity on fibroblast cells. The scaffold also showed good blood compatibility according to the effects of coagulation time. As well as significant fibroblast migration and proliferation on the wound margin, according to wound‐healing assay. All in all, the developed biocompatible, antioxidant, and antibacterial Ag‐ZnO NPs incorporated CS/PEO nanofibrous mats showed their potential as an effective wound dressing.