Open AccessThe spatiotemporal localization of JAM‐C following sciatic nerve crush in adult rats
Author(s) -
Avari Parizad,
Huang Wenlong,
Averill Sharon,
Colom Bartomeu,
Imhof Beat A.,
Nourshargh Sussan,
Priestley John V.
Publication year - 2012
Publication title -
brain and behavior
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.915
H-Index - 41
ISSN - 2162-3279
DOI - 10.1002/brb3.63
Subject(s) - sciatic nerve , remyelination , anatomy , crush injury , nerve injury , myelin , medicine , central nervous system , anesthesia , surgery
JAM‐C is a junctional adhesion molecule, enriched at tight junctions on endothelial and epithelial cells, and also localized to Schwann cells at junctions between adjoining myelin end loops. The role of JAM‐C following peripheral nerve injury (PNI) is currently unknown. We examined the localization of JAM‐C after sciatic nerve crush injury in adult rats. JAM‐C immunoreactivity was present in paranodes and incisures in sham surgery control nerve, but distal to the crush injury significantly decreased at three and 14 days. JAM‐C was re‐expressed at 28 days and, by 56 days, was significantly increased in the distal nerve compared to controls. In a 7‐mm length of sciatic nerve sampled distal to the crush site, the densities of JAM‐C immunoreactive paranodes increased in the distal direction. Conversely, the densities of JAM‐C immunoreactive incisures were highest immediately distal to the crush site and decreased in the more distal direction. Further analysis revealed a strong correlation between JAM‐C localization and remyelination. Fifty‐six days after crush injury, greater densities of JAM‐C paranodes were seen compared to the nodal marker jacalin, suggesting that paranodal JAM‐C precedes node formation. Our data are the first to demonstrate a potential role of JAM‐C in remyelination after PNI.