Open AccessMild hypothermia improves brain injury in rats with intracerebral hemorrhage by inhibiting IRAK2/NF‐κB signaling pathway
Author(s) -
Shi Hui,
Su Zulu,
Su Hai,
Chen Hao,
Zhang Yi,
Cheng Yuan
Publication year - 2021
Publication title -
brain and behavior
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.915
H-Index - 41
ISSN - 2162-3279
DOI - 10.1002/brb3.1947
Subject(s) - proinflammatory cytokine , nissl body , medicine , apoptosis , intracerebral hemorrhage , hypothermia , pharmacology , western blot , neuroprotection , oxidative stress , anesthesia , inflammation , pathology , chemistry , staining , subarachnoid hemorrhage , biochemistry , gene
Objective To explore the effect of mild hypothermia on nerve injury by establishing a rat model of intracerebral hemorrhage (ICH), and to clarify the specific molecular mechanism of mild hypothermia in improving brain injury in ICH rats. Methods The rat model of ICH was established by collagenase injection. The neurological deficit score (NDS), brain tissue water detection, and Nissl staining were applied to detect the degree of brain injury. ELISA was used to analyze the expression of proinflammatory cytokines and serum nerve injury indexes. Flow cytometry and Western Blot were used to detect neuronal apoptosis. Results Mild hypothermia treatment significantly improved the brain injury of the ICH rats and down‐regulated the inflammatory response and oxidative stress in the brain tissue. Moreover, mild hypothermia also effectively inhibited IRAK2/NF‐κB signaling pathway and thus affect neuronal apoptosis. Conclusion Mild hypothermia alleviates inflammatory response and neuronal apoptosis by inhibiting IRAK2/NF‐κB signaling pathway in the ICH rats thus improving brain injury.