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Structural characterization of synthetic model peptides of the DNA‐binding cI434 repressor by electrospray ionization and fast atom bombardment mass spectrometry
Author(s) -
Percipalle Piergiorgio,
Saletti Rosaria,
Pongor Sandor,
Foti Salvatore,
Tossi Alessandro,
Fisichella Salvatore
Publication year - 1994
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200231203
Subject(s) - chemistry , fast atom bombardment , mass spectrometry , electrospray ionization , protein mass spectrometry , chromatography , peptide , characterization (materials science) , electrospray , biochemistry , materials science , nanotechnology
The structural characterization of two synthetic model peptides of the cI434 repressor is described. Unequivocal determination of the structure was achieved by means of electrospray ionization mass spectrometry of the intact peptides and by fast atom bombardment mass spectrometric identification of complementary peptide fragments obtained by tryptic and chymotrypic digestion and partial separation by reversed‐phase high‐performance liquid chromatography. The results show the potential of this approach for characterizing synthetic peptides of relatively high molecular weight.