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Optimization of the analytical performance of the magnetic sector mass spectrometer for the identification of residual chloramphenicol in shrimp
Author(s) -
Bencsath F. Aladar,
Plakas Steven M.,
Long Austin R.
Publication year - 1994
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200231104
Subject(s) - mass spectrometry , analyte , analytical chemistry (journal) , ionization , resolution (logic) , electron ionization , chemistry , residual , shrimp , detection limit , noise (video) , chromatography , ion , mathematics , organic chemistry , algorithm , artificial intelligence , fishery , computer science , image (mathematics) , biology
Chemical noise limits mass spectrometric detection of chloramphenicol (CAP) with electron capture ionization at low resolution, and makes CAP identification at concentrations of 5 parts per billion (ppb) difficult. Increasing the resolution from 1000 to 3500, however, was sufficient to separate the analyte signals from the noise signals, and resulted in a 100 times higher analytical sensitivity. The introduction of sweep gas in the ion source decreased the scattering of the quantitative results on average by a factor of 7, and thereby improved the precision of the analyses to an acceptable level (CV < 10%). Under such conditions, CAP residues of 1.5 and 2.1 ppb in shrimp as determined by electron capture gas chromatography/mass spectrometry can readily be identified by monitoring four diagnostic ions.