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Study on the decomposition of the amadori compound N ‐(1‐deoxy‐D‐fructos‐1‐y1)‐glycine in model systems: Quantification by fast atom bombardment tandem mass spectrometry
Author(s) -
Staempfli Andreas A.,
Blank Imre,
Fumeaux René,
Fay Laurent B.
Publication year - 1994
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200231007
Subject(s) - chemistry , fast atom bombardment , derivatization , mass spectrometry , decomposition , amadori rearrangement , tandem mass spectrometry , glycine , isotope dilution , tandem , chromatography , analytical chemistry (journal) , amino acid , organic chemistry , biochemistry , materials science , receptor , glycation , composite material
A new method for the quantification of N ‐(1‐deoxy‐D‐fructos‐1‐yl)‐glycine (DFG) was developed based on isotope dilution fast atom bombardment tandem mass spectrometry using 13 C‐labelled DFG as an internal standard. This method, which requires neither derivatization nor clean‐up of the samples, was used to study the degradation of DFG under different conditions (time and pH). It was found that the decomposition of DFG at 90°C was favoured at pH 7 compared to pH 6. The higher stability of DFG at pH 6 was due to 1,2‐enolization leading to the relatively stable 3‐deoxglucosone.