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Open‐chain peptides obtained by acidic hydrolytic cleavage of cyclosporin A
Author(s) -
Magni F.,
Arcelloni C.,
Paroni R.,
Fermo I.,
Bonini P. A.,
Del Puppo M.,
Manzocchi A.,
Kienle M. Galli
Publication year - 1994
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200230809
Subject(s) - hydrolysis , chemistry , cleavage (geology) , fast atom bombardment , chromatography , residue (chemistry) , mass spectrometry , tandem mass spectrometry , acid hydrolysis , ether , stereochemistry , organic chemistry , geotechnical engineering , fracture (geology) , engineering
Hydrolysis of cyclosporin A (CsA) was studied in order to clarify the still undefined point of attack of the acidic degradation. Among ether extractable and water‐soluble products formed from CsA in HCl, two open‐chain peptides were isolated by high‐performance liquid chromatography which were identified as the deca‐ and nonapeptides deriving from CsA through the hydrolytic cleavage of amino acid residue 11 and both residues 11 and 10, respectively. Identification was carried out by fast atom bombardment tandem mass spectrometry.