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Measurement of whole body acetate turnover in healthy subjects with stable isotopes
Author(s) -
Simoneau C.,
Pouteau E.,
Maugeais P.,
Marks L.,
Ranganathan S.,
Champ M.,
Krempf M.
Publication year - 1994
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200230707
Subject(s) - propionate , chemistry , isotope , stable isotope ratio , fermentation , tracer , flux (metallurgy) , chromatography , radiochemistry , biochemistry , organic chemistry , physics , quantum mechanics , nuclear physics
Abstract Colonic fermentation of dietary fibres produces short‐chain fatty acids (e.g. acetate, propionate). Measurements of whole body acetate turnover was used in order to estimate the production of colonic short‐chain fatty acids in human subjects. However, higher flux rates for acetate have been reported in human studies with stable isotopes as compared to radioactive tracers. The reasons for this discrepancy are unclear. In this study, the stable isotope (1‐ 13 C)acetate was used and a method was developed to measure its enrichment in plasma. Variations between and within assays were less than 5%. The standard curve was linear from 0.5% to 10% enrichment. When this tracer was infused for 160 min in six healthy volunteers, acetate turnover was found to be 7.5 ± 1 μmol kg −1 min −1 , which is similar to data reported with radioactive tracers. We assumed that the higher flux rate previously observed with stable isotope tracers was related to differences in the physiological status of the subjects involved in these studies.