Premium
Characterization of fluorinated ethylchloroformate derivatives of protein amino acids using positive and negative chemical ionization gas chromatography/mass spectrometry
Author(s) -
Vatankhah Mahnaz,
Moini Mehdi
Publication year - 1994
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200230507
Subject(s) - chemistry , chemical ionization , mass spectrometry , chromatography , pyridine , amino acid , ethyl ester , gas chromatography , gas chromatography–mass spectrometry , organic chemistry , ion , ethanol , ethyl chloroformate , carboxylic acid , ionization , biochemistry
A solution of ethyl chloroformate + trifluoroethanol (or ethanol) + pyridine reacts rapidly with carboxylic and amino groups of amino acids to form n‐ethoxycarbonyl amino acid trifluoroethyl (or ethyl) esters, which produce strong peaks representative of molecular ions in both positive and negative chemical ionization gas chromatography/mass spectrometry. Sensitivity of detection of the trifluoroethyl ester derivatives versus ethyl ester derivatives in the positive and negative chemical ionization modes is also discussed.