Mass spectrometric studies on the  N ‐linked oligosaccharides of baculovirus‐expressed mouse interleukin‐3 | Zendy

Hogeland Kenneth E. | Zendy; Deinzer Max L. | Zendy

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Mass spectrometric studies on the N ‐linked oligosaccharides of baculovirus‐expressed mouse interleukin‐3

Author(s) -

Hogeland Kenneth E.,

Deinzer Max L.

Publication year - 1994

Publication title -

biological mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.475

H-Index - 121

eISSN - 1096-9888

pISSN - 1052-9306

DOI - 10.1002/bms.1200230406

Subject(s) - chitobiose , oligosaccharide , chemistry , mass spectrometry , recombinant dna , lymphokine , spodoptera , biochemistry , chromatography , microbiology and biotechnology , biology , chitin , in vitro , chitosan , gene

A combination of mass spectrometry techniques, high‐pH anion‐exchange chromatography and enzymatic digestions has been applied to elucidate the structures of the N ‐linked oligosaccharides on baculovirus‐expressed mouse interleukin‐3 produced in Bombyx mori larvae. This recombinant lymphokine was found to be heterogeneously glycosylated with mannosidic chitobiose core type oligosaccharides. A single N ‐linked oligosaccharide with the truncated structure of Manα1‐6Manβ1‐4GlcNAc1‐4(Fucα1‐6)GlcNAc was found to be the predominant species.

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