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Mass spectrometric studies on the N ‐linked oligosaccharides of baculovirus‐expressed mouse interleukin‐3
Author(s) -
Hogeland Kenneth E.,
Deinzer Max L.
Publication year - 1994
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200230406
Subject(s) - chitobiose , oligosaccharide , chemistry , mass spectrometry , recombinant dna , lymphokine , spodoptera , biochemistry , chromatography , microbiology and biotechnology , biology , chitin , in vitro , chitosan , gene
A combination of mass spectrometry techniques, high‐pH anion‐exchange chromatography and enzymatic digestions has been applied to elucidate the structures of the N ‐linked oligosaccharides on baculovirus‐expressed mouse interleukin‐3 produced in Bombyx mori larvae. This recombinant lymphokine was found to be heterogeneously glycosylated with mannosidic chitobiose core type oligosaccharides. A single N ‐linked oligosaccharide with the truncated structure of Manα1‐6Manβ1‐4GlcNAc1‐4(Fucα1‐6)GlcNAc was found to be the predominant species.