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Femtogram on‐column detection of nicotine by isotope dilution gas chromatography/negative ion detection mass spectrometry
Author(s) -
Cooper Donald A.,
Moore James M.
Publication year - 1993
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200221006
Subject(s) - isotope dilution , chemistry , chromatography , mass spectrometry , gas chromatography , gas chromatography–mass spectrometry , ion mobility spectrometry–mass spectrometry , analytical chemistry (journal) , ion , selected reaction monitoring , tandem mass spectrometry , organic chemistry
A gas chromatographic method, utilizing detection by negative chemical ionization (NCI) mass spectrometry, is presented for the detection of nicotine. The method incorporates derivatization with heptafluorobutyric anhydride/pyridine to yield the ring‐opened addition product, 1‐[4‐(3′‐pyridyl)‐4‐heptafluorobutyryloxybutyl]‐1‐methylheptafluorobutanamide. The resulting heptafluorobutanamide is both relatively stable and an excellent electron scavenger. This methodology provides for the detection of nicotine at femtogram on‐column levels, the measurement of nicotine over three orders of magnitude, and the minimization of contamination due to environmental nicotine.