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Reproducibility and quantitation of matrix‐assisted laser desorption ionization mass spectrometry: Effects of nitrocellulose on peptide ion yields
Author(s) -
Preston L. M.,
Murray K. K.,
Russell D. H.
Publication year - 1993
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200220908
Subject(s) - reproducibility , mass spectrometry , nitrocellulose , chemistry , chromatography , ion suppression in liquid chromatography–mass spectrometry , matrix (chemical analysis) , analytical chemistry (journal) , ion , ionization , desorption , tandem mass spectrometry , biochemistry , membrane , organic chemistry , adsorption
We have observed that using nitrocellulose in sample preparation for matrix‐assisted laser desorption ionization (MALDI) time‐of‐flight mass spectrometry increases the yield of peptide [M + H] + ions. Addition of nitrocellulose also provides improvement in sample‐to‐sample reproducibility of MALDI ion yield and improves the precision of peptide quantitation. Mass spectrometry and optical microscopy results suggest that the nitrocellulose modifies the crystallization of matrix/analyte solution to allow more even coverage over the sample surface.