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Collision‐induced decomposition of sphingomyelins for structural elucidation
Author(s) -
Ann Qinghong,
Adams Jeaneatte
Publication year - 1993
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200220504
Subject(s) - chemistry , phosphorylcholine , sphingomyelin , derivatization , decomposition , ion , fast atom bombardment , chromatography , ceramide , mass spectrometry , organic chemistry , membrane , biochemistry , apoptosis
Fast atom bombardment (FAB) and collision‐induced decomposition (CID) are used to determine the structures of sphingomyelins. CID of [M + H] + ions; [M + Cat] + ions, in which Cat = Li + , Na + , K + , Rb + or Cs + ; and [M – 15] − , [M – 60] − , and [M – 86] − ions are investigated. CID of the anions reveals fragmentations that allow simultaneous characterization of both the ceramide and the phosphorylcholine portions of sphingomyelins. Consequently, information regarding the lengths of the sphingoid base and fatty acyl chain, and the locations of double bonds on the fatty acyl chain can be directly obtained. Structures of individual components of complex mixtures can also be determined without prior chemical derivatization or gas chromatographic separation. The detection limits for obtaining acceptable FAB and CID spectra of the [M – 15] − ions of N ‐oleoyl‐(4 E )‐sphingenine phosphorylcholine are 4 and 30 pmol, respectively.