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Determination of amphetamine, methamphetamine and desmethyldeprenyl in human plasma by gas chromatography/negative ion chemical ionization mass spectrometry
Author(s) -
Reimer Mark L. J.,
Mamer Orval A.,
Zavitsanos A. Paul,
Siddiqui A. Waheed,
Dadgar D.
Publication year - 1993
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200220404
Subject(s) - chemistry , chromatography , mass spectrometry , chemical ionization , methamphetamine , analyte , isotope dilution , gas chromatography , gas chromatography–mass spectrometry , amphetamine , ion , electron ionization , selected ion monitoring , ionization , organic chemistry , medicine , neuroscience , dopamine , biology
A sensitive and specific assay for the quantitative determination of amphetamine, methamphetamine and desmethyldeprenyl in human plasma specimens is described. Electron capture/negative ion chemical ionization gas chromatography/mass spectrometry is used to determine the extracted plasma concentrations of the three target compounds as their N ‐heptafluorobutyryl derivatives. Quantitation is performed by stable isotope dilution using d 6 ‐amphetamine and d 6 ‐methamphetamine as internal standards. Selected ion monitoring of the [M – HF] − ions of both the analytes and internal standards results in minimum quantifiable limits of 0.10 ng ml −1 for both amphetamine and methamphetamine and 0.25 ng ml −1 for desmethyldeprenyl. Excellent linearity ( r = 0.998) up to at least 5.00 ng ml −1 is demonstrated.