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Study of intracellular deposition of the anti‐leprosy drug Clofazimine in mouse spleen using laser microprobe mass analysis
Author(s) -
Vandeputte D.,
Jacob W.,
Van Grieken R.,
Boddingius J.
Publication year - 1993
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200220402
Subject(s) - clofazimine , microprobe , spleen , mass spectrum , chemistry , leprosy , mass spectrometry , analytical chemistry (journal) , intracellular , pathology , chromatography , biology , medicine , mineralogy , biochemistry , immunology
Laser microprobe mass analysis (LAMMA) was used to study the composition of the brick‐red crystalline material which had accumulated in the spleen of mice that had received the anti‐leprosy drug Clofazimine in their diet for several months. The crystalline deposits light‐microscopically resembled pure Clofazimine crystals. The presence of the drug in the crystals was indicated by LAMMA by the appearance of the chloride mass peaks in the negative mass spectra. More specific information was obtained from the positive mass spectra. A mass signal for the protonated molecule was present.