Premium
Mass spectrometry in the integrated strategy for the structural analysis of protein variants
Author(s) -
Wada Yoshinao
Publication year - 1992
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200211202
Subject(s) - mass spectrometry , protein mass spectrometry , chemistry , tandem mass spectrometry , electrospray ionization , top down proteomics , fast atom bombardment , tandem mass tag , isobaric labeling , sample preparation in mass spectrometry , mass spectrometry imaging , dna , proteomics , computational biology , chromatography , gene , biochemistry , quantitative proteomics , biology
A significant proportion of genetic disorders are caused by point mutations in proteins, and many variants of haemoglobin have been characterized by mass spectrometry. The strategy employs fast atom bombardment and/or tandem mass spectrometry of enzymatically digested peptides for elucidating the type and position of the mutations. Electrospray ionization mass spectrometry is useful for detecting mutations in the intact protein molecule, but the competence diminishes with increasing mass or increasing number of 13 C atoms. Recent developments of DNA techniques requires us to re‐evaluate the status of mass spectrometry in this field. The role of mass spectrometry in the structural analysis of protein variants is discussed, and is compared with chemical methods and DNA strategies.