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The sensitive determination of abanoquil in blood by high‐performance liquid chromatography/atmospheric pressure ionization mass spectrometry
Author(s) -
Kaye B.,
Clark M. W. H.,
Cussans N. J.,
Macrae P. V.,
Stopher D. A.
Publication year - 1992
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200211110
Subject(s) - chromatography , mass spectrometry , atmospheric pressure chemical ionization , direct electron ionization liquid chromatography–mass spectrometry interface , chemistry , tandem mass spectrometry , high performance liquid chromatography , chemical ionization , atmospheric pressure , ionization , analytical chemistry (journal) , liquid chromatography–mass spectrometry , selected reaction monitoring , ion , oceanography , organic chemistry , geology
A method is described for the determination of abanoquil in human blood. The method is based on high‐performance liquid chromatography(HPLC)/atmospheric pressure positive ion chemical ionization mass spectrom‐etry, using ( 2 H 3 )abanoquil as internal standard. Multiple reaction monitoring is employed for selectivity and sensitivity, which enables quantification over the range 10–500 pg ml −1 with acceptable precision and accuracy. This assay methodology illustrates the versatility of atmospheric pressure ionization/tandem mass spectrometry, in conjunction with HPLC, for the separation and quantification of drugs in the subnanogram per millilitre range.