Premium
Analysis of peptides of human seminal plasma by mass spectrometry
Author(s) -
Kausler Wieland,
Spiteller Gerhard
Publication year - 1992
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200211108
Subject(s) - mass spectrometry , chemistry , peptide , residue (chemistry) , chromatography , cleavage (geology) , human plasma , protein mass spectrometry , peptide sequence , tandem mass spectrometry , biochemistry , biology , paleontology , fracture (geology) , gene
A procedure is described to isolate peptides from complex biological fluids (e.g. seminal plasma) in pure form within a short time. The sequence of the isolated peptides was determined by liquid secondary ion mass spectrometry in combination with enzymatic cleavage reactions. The method was used for the structure elucidation of a peptide of molecular weight 2766 u with an N‐terminal pyroglutamine residue.