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Mass spectrometric quantification of cysteine‐containing leukotrienes in rat bile using 13 C‐labeled internal standards
Author(s) -
Raftery Mark J.,
Justesen Ulla,
Jaeschke Hartmut,
Gaskell Simon J.
Publication year - 1992
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200211008
Subject(s) - chemistry , chromatography , leukotriene , trimethylsilyl , leukotriene e4 , mass spectrometry , detection limit , fast atom bombardment , leukotriene c4 , ether , tandem mass spectrometry , fractionation , organic chemistry , medicine , asthma
Leukotriene C 4 and N ‐acetyl leukotriene E 4 were determined in rat bile using a procedure based on high‐performance liquid chromatographic fractionation, hydrogenation to 5‐hydroxyeicosanoic acid, and gas chomatographic/mass spectrometric selected ion monitoring analysis of the pentafluorobenzyl ester, trimethylsilyl ether derivatives. 13 C 4 ‐Labeled analogs of the leukotrienes were synthesized and used as internal standards. The concentrations of both leukotrienes in rat bile were markedly elevated after administration of endotoxin to anesthetized animals; N ‐acetyl leukotriene E 4 was the more abundant product. The presence of leukotriene C 4 in rat bile after endotoxin challenge was confirmed by fast atom bombardment/tandem mass spectrometry with precursor ion scanning. Quantitative determination of leukotriene C 4 using the tandem mass spectrometric procedure was consistent wth the gas chromatographic/mass spectrometric data but the latter procedure gave a substantially lower detection limit.