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Fast atom bombardment mass spectrometry of sulphated metabolites of the steroid org OD 14 using a glycerol matrix saturated with tetramethylammonium hydroxide
Author(s) -
Jacobs Peter L.,
Schmeits Gerrit J. H.,
De Vries Marcel P.,
Delbressine Leon P. C.,
Peters Harry A. M.
Publication year - 1992
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200211005
Subject(s) - tetramethylammonium hydroxide , chemistry , tetramethylammonium , glycerol , ion , fragmentation (computing) , hydroxide , fast atom bombardment , steroid , mass spectrometry , matrix (chemical analysis) , mass spectrum , matrix isolation , inorganic chemistry , chromatography , analytical chemistry (journal) , organic chemistry , infrared spectroscopy , biochemistry , hormone , operating system , computer science
The use of a glycerol matrix saturated with tetramethylammonium hydroxide pentahydrate allowed the recording of distinct positive and negative ion fast atom bombardment (FAB) spectra of sulphated metabolites of the steroid Org OD 14 in the presence of sodium acetate where glycerol as the FAB matrix failed. From the combined positive and negative ion FAB spectra of the ion‐pair complexes the number of sulphate groups present could be ‘counted’. Due to the presence of characteristic satellite ions (loss of C 2 H 2 , a novel charge‐remote fragmentation) in the positive ion spectra of the 17β‐hydroxy, 17α‐ethynyl steroids, metabolites sulphated at the 3‐position could be distinguished from compounds sulphated at the 17β‐position.