Premium
Determination of multiple selenium stable isotope tracers by inductively coupled plasma mass spectrometry
Author(s) -
Buckley Wayne T.,
Budac James J.,
Godfrey David V.,
Koenig Karen M.
Publication year - 1992
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200211002
Subject(s) - isotope dilution , isotope , chemistry , tracer , inductively coupled plasma mass spectrometry , selenium , stable isotope ratio , mass spectrometry , radiochemistry , analytical chemistry (journal) , chromatography , physics , organic chemistry , quantum mechanics , nuclear physics
Inductively coupled plasma mass spectrometry with hydride generation was used for the determination of Se stable isotope tracer enrichment in standard solutions and biological materials. Up to three enriched isotopes, 76 Se, 77 Se and 82 Se, were determined, while 78 Se served as the reference isotope. Tracer enrichment was linearly determined with or without simultaneous additions of two other Se stable isotope tracers. Multiple additions of enriched 77 Se and enriched 82 Se at the low levels required for in vivo tracers were accurately determined in the presence of a much greater addition of enriched 76 Se (for isotope dilution analysis) in spite of the significant 77 Se and 82 Se impurities introduced with the enriched 76 Se. Detection limits for Se stable isotope tracers were sufficient to determine up to a 200‐fold dilution of tracer following an initial tracer enrichment equivalent to 10% of the mass of the natural Se pool.