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Design and optimization of a micropump‐based electrospray device for preparation of nitrocellulose substrates for plasma desorption time‐of‐flight mass spectrometry
Author(s) -
Bondarenko P. V.,
Zubarev R. A.,
Knysh A. N.,
Rozynov B. V.
Publication year - 1992
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200210702
Subject(s) - micropump , mass spectrometry , nitrocellulose , desorption , electrospray , materials science , plasma , time of flight mass spectrometry , analytical chemistry (journal) , chromatography , nanotechnology , chemistry , ion , physics , adsorption , nuclear physics , biochemistry , membrane , ionization , organic chemistry
Details of a nitrocellulose (NC) substrate preparation technique in 252 Cf plasma desorption time‐of‐flight mass spectrometry were investigated using an electrospray device, in which a micropump was employed for solution delivery. The molecular ion yields for three standard proteins (porcine insulin, chicken‐egg lysozyme and chymotrypsinogen A) were studied as a function of the electrosprayed NC layer thickness, spray rate, NC solution concentration and some other parameters. Optimal parameters of the NC substrate preparation procedure were determined, which include deposition of a layer of 250–750 μg cm −2 at up to 15 μl min −1 spray rate and up to 15 μg μl −1 solution concentration.