Design and optimization of a micropump‐based electrospray device for preparation of nitrocellulose substrates for plasma desorption time‐of‐flight mass spectrometry | Zendy

Bondarenko P. V. | Zendy; Zubarev R. A. | Zendy; Knysh A. N. | Zendy; Rozynov B. V. | Zendy

Premium

Design and optimization of a micropump‐based electrospray device for preparation of nitrocellulose substrates for plasma desorption time‐of‐flight mass spectrometry

Author(s) -

Bondarenko P. V.,

Zubarev R. A.,

Knysh A. N.,

Rozynov B. V.

Publication year - 1992

Publication title -

biological mass spectrometry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.475

H-Index - 121

eISSN - 1096-9888

pISSN - 1052-9306

DOI - 10.1002/bms.1200210702

Subject(s) - micropump , mass spectrometry , nitrocellulose , desorption , electrospray , materials science , plasma , time of flight mass spectrometry , analytical chemistry (journal) , chromatography , nanotechnology , chemistry , ion , physics , adsorption , nuclear physics , biochemistry , membrane , ionization , organic chemistry

Details of a nitrocellulose (NC) substrate preparation technique in 252 Cf plasma desorption time‐of‐flight mass spectrometry were investigated using an electrospray device, in which a micropump was employed for solution delivery. The molecular ion yields for three standard proteins (porcine insulin, chicken‐egg lysozyme and chymotrypsinogen A) were studied as a function of the electrosprayed NC layer thickness, spray rate, NC solution concentration and some other parameters. Optimal parameters of the NC substrate preparation procedure were determined, which include deposition of a layer of 250–750 μg cm −2 at up to 15 μl min −1 spray rate and up to 15 μg μl −1 solution concentration.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research