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Analysis of disaccharides by periodate oxidation and mass spectrometry
Author(s) -
Angel AnneSophie,
Nilsson Bo
Publication year - 1992
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200210405
Subject(s) - periodate , chemistry , pentose , disaccharide , glycosidic bond , mass spectrometry , sodium periodate , fast atom bombardment , organic chemistry , chromatography , fermentation , enzyme
A method for the analysis of disaccharides by mass spectrometry is described. Disaccharides and disaccharide alditols were subjected to periodate oxidation and reduction and the products were analysed by electron ionization and fast atom bombardment mass spectrometry as peracetylated and permethylated derivatives. Disaccharides with a reducing terminal hexose or pentose were oxidized as reducing compounds, whereas compounds with an N ‐acetylhexosamine in the reducing position were reduced prior to the periodate oxidation. The compounds gave simple mass spectra with intense and characteristic ions from which glycosidic linkage positions and ring size could be determined.