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Identification of AJ‐2615 and its S ‐oxidized metabolites in rat plasma by use of tandem‐mass spectrometry
Author(s) -
Kurono Masuo,
Itogawa Akira,
Yoshida Koji,
Naruto Shunsuke,
Rudewicz Patrick,
Kanai Michiko
Publication year - 1992
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200210105
Subject(s) - chemistry , mass spectrometry , tandem mass spectrometry , chromatography , diastereomer , tandem , plasma , fast atom bombardment , ion , infrared spectroscopy , stereochemistry , organic chemistry , materials science , physics , quantum mechanics , composite material
The structural elucidation of the metabolites of a new calcium entry blocker, AJ‐2615 (AJ), in rat plasma is described. Metabolites in a crude plasma extract from spontaneously hypertensive rats were identified without chromatographic separation by fast atom bombardment tandem mass spectrometry. When the plasma extract was examined by using parent ion scans, the presence of the oxidized metabolites of AJ was suggested. These metabolites were identified as the S ‐oxide and the S,S ‐dioxide by comparing their daughter ion spectra with those of authentic samples. The presence of the two diastereomeric S ‐oxides of AJ in the plasma extract was ascertained by high‐performance liquid chromatography. Their relative configurations were determined by infrared and proton nuclear magnetic resonance spectra.