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Coaxial continuous flow fast atom bombardment for higher‐molecular‐weight peptides: Comparison with static fast atom bombardment and electrospray ionization
Author(s) -
Tomer Kenneth B.,
Perkins John R.,
Parker Carol E.,
Deterding Leesa J.
Publication year - 1991
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200201207
Subject(s) - coaxial , fast atom bombardment , analytical chemistry (journal) , electrospray ionization , ionization , mass spectrometry , chemistry , electrospray , atom (system on chip) , continuous flow , atomic physics , chromatography , physics , ion , organic chemistry , mechanics , electrical engineering , engineering , computer science , embedded system
A comparison of coaxial continuous flow fast atom bombardment (FAB) with static FAB and with electrospray ionization (ESI) for the analysis of ‘high’‐mass peptides ( M r = 3000–4000) is presented. Sensitivities of the peptides by coaxial continuous flow FAB is nearly an order of magnitude better than by static FAB. Single‐scan spectra with good signal‐to‐noise can be obtained from as little as 200 fmol (by flow injection analysis). Detection limits by ESI mass spectrometry were found to be equivalent to 20 times higher than by coaxial continuous flow FAB on a per mole basis, but 4–20 times lower on a concentration basis, owing to the greater flow per unit time employed in the ESI mass spectrometric experiments.