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Determination of nitecapone and ( 13 C 6 )nitecapone in human plasma by gas chromatography/mass spectrometry
Author(s) -
Ottoila Pekka,
Pakkala Eila,
Karlsson Carita,
Taskinen Jyrki
Publication year - 1991
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200201205
Subject(s) - chromatography , analyte , gas chromatography , chemistry , pharmacokinetics , mass spectrometry , gas chromatography–mass spectrometry , coefficient of variation , pharmacology , medicine
A gas chromatographic/mass spectrometric method is developed and validated for simultaneous determination of nitecapone and its 13 C 6 ‐labelled analogue in human plasma using ( 2 H 6 , 13 C 6 )nitecapone as internal standard. The method involves extraction of the analytes from plasma to ethyl acetate‐hexane mixture (20:80) and conversion to bis(trimethylsilyl) ethers prior to determination by gas chromatography/mass spectrometry using selected ion monitoring. The quantification range is 0.5–2000 ng ml −1 . Precision ranges from 11.3% (coefficient of variation) at low levels to 2.4% at high levels. Recovery is about 50% in the whole range. The method is applied to a pharmacokinetic study where nitecapone diluted with 14 C‐labelled nitecapone is given intravenously concomitantly with an oral dose of ( 13 C 6 )nitecapone.