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Isotope dilution mass spectrometry for procaine determination in biological samples
Author(s) -
Culea Monica,
Palibroda Nicolae,
Chiriac Maria,
Moldovan Zaharie,
Abraham Alexandru D.,
Frangopol Petre T.
Publication year - 1991
Publication title -
biological mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1052-9306
DOI - 10.1002/bms.1200201114
Subject(s) - isotope dilution , chromatography , mass spectrometry , chemistry , procaine , dilution , isotope , resolution (logic) , analytical chemistry (journal) , accuracy and precision , mathematics , pharmacology , medicine , physics , statistics , quantum mechanics , artificial intelligence , computer science , thermodynamics
A simple, rapid and sensitive method for procaine determination is described. Isotope dilution mass spectrometry with ( 15 N)procaine as internal standard was used. The analysis was performed at 4000 resolution by selected ion monitoring with temperature programming. The sample was measured in underivatized form in the direct inlet system. The method shows good analytical parameters: linearity between 0 and 40 μg ml −1 , good precision and accuracy. The method was applied to the in vitro pharmacokinetic study of the metabolism of procaine in liver homogenates of Wistar rats. The method is rapid, permitting about six samples to be run per hour. Sensitivity of the method permits analysis at a signal‐to‐noise ratio of 5:1.